当前位置:首页 >> 生命科学 >> 微生物学
基因改造使大肠杆菌杀灭绿脓杆菌

病菌对抗生素产生了耐药性怎么办?或许可以用另一种细菌来杀死它。新加坡研究人员利用合成生物学手段,通过基因改造使大肠杆菌分泌专门的毒素,成功杀死绿脓杆菌。

绿脓杆菌是一种生存能力很强、对多种抗生素和消毒剂有耐药性的细菌。它对健康人的影响较小,但对免疫力低下的人群有很大威胁,是导致医院感染的主要菌种之一。

不同类型的绿脓杆菌之间因生存竞争而存在“内战”,它们会分泌称为绿脓菌素的毒素,彼此攻击。每种绿脓菌素只对特定菌株起作用。新加坡南洋理工大学的研究人员利用这种特性,给大肠杆菌植入基因,使其分泌可杀灭感染人类的绿脓杆菌菌株的毒素。

在这种大肠杆菌作用下,实验室中培养的绿脓杆菌只有1%能够存活,绿脓杆菌形成的生物膜也比正常情况下稀薄得多。生物膜是由细菌及其分泌的物质形成的膜状物,毒性和耐药性比单个细菌更强。

相关成果发表在新一期的英国《分子系统生物学》杂志上。研究人员说,这是将合成生物学应用于抗感染的第一个例证。

不过这一方法目前还有缺陷,离实用尚有距离。例如,目前该方法只有在大肠杆菌数量比绿脓杆菌多几倍时才有效,而很多情况下不宜往感染部位大量添加细菌。

研究小组正在设法改进这种方法,并在培养另一种大肠杆菌菌株,用于杀灭霍乱弧菌。

英文论文摘要:

Engineering microbes to sense and eradicatePseudomonas aeruginosa, a human pathogen

Abstract

Synthetic biology aims to systematically design and construct novel biological systems that address energy, environment, and health issues. Herein, we describe the development of a synthetic genetic system, which comprises quorum sensing, killing, and lysing devices, that enables Escherichia coli to sense and kill a pathogenic Pseudomonas aeruginosa strain through the production and release of pyocin. The sensing, killing, and lysing devices were characterized to elucidate their detection, antimicrobial and pyocin release functionalities, which subsequently aided in the construction of the final system and the verification of its designed behavior. We demonstrated that our engineered E. coli sensed and killed planktonic P. aeruginosa, evidenced by 99% reduction in the viable cells. Moreover, we showed that our engineered E. coli inhibited the formation of P. aeruginosa biofilm by close to 90%, leading to much sparser and thinner biofilm matrices. These results suggest that E. coli carrying our synthetic genetic system may provide a novel synthetic biology-driven antimicrobial strategy that could potentially be applied to fighting P. aeruginosa and other infectious pathogens.

 

分享按钮
评论:   
验证码:  
  [Ctrl+Enter]
相关文章
古老蛋白塑造细菌紧凑基因组
两种常用大肠杆菌菌株基因组测序完成
美开发出肠毒性大肠杆菌疫苗
大肠杆菌捕获糖的机制新发现
羟基脲杀死细菌的分子机制
寻找有效的大肠杆菌疫苗
基因时钟让大肠杆菌细胞同步
摘要
关键字
大肠杆菌 绿脓杆菌
 
 
最新推荐文章


近期热点新闻