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miR-210的失调可能在狼疮和类风湿发病机制中发挥重要作用

摘要


背景:在缺氧条件,miRNA-210调节缺氧诱导因子-1α表达起着重要的作用(HIF-1α)和辅助性T细胞17(Th17)细胞分化,这可能与免疫系统的发育和功能相关。


目的:本研究旨在探讨外周血单个核细胞(PBMC)miR-210表达水平与系统性红斑狼疮(SLE)、类风湿性关节炎(RA)和两种疾病的临床和实验室特点的关系。


方法:实时定量逆转录聚合酶链反应(RT PCR)法检测外周血单个核细胞miR-210表达水平35例SLE患者,38例RA患者、35例健康对照。


结果:与健康对照组比较,SLE患者miR-210表达水平显著增加(P = 0.001)。miR-210 SLE胸膜炎(Z = -2.345,P = 0.019)和anti-SSB/La抗体阳性组(Z = -2.076,P = 0.038)。然而,我们没有发现miR-210的水平与系统性红斑狼疮疾病活动指数(SLEDAI)之间的显著相关性(r = 0.091分,P = 0.602)。虽然,在RA患者中miR-210的水平与对照组发现无显著差异(Z = -1.226,P = 0。220)。有一个显着的表达降低,在活动期RA患者miR-210比不运动的RA患者(Z = -4.011,P<0.001)。


结论:在SLE和RA患者miR-210的水平表明miR-210的失调可能在这些疾病的发病机制中起重要作用。


Abstract


BACKGROUND:In hypoxic conditions, miRNA-210 plays an important role in regulating the expression of hypoxia-inducing factor-1α (HIF-1α) and the differentiation of T helper 17 (Th17) cells, and this may be involved in the development and function of the immune system.


AIMS:This study was to investigate the miR-210 expression levels in peripheral blood mononuclear cells (PBMCs) from patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and its association with the clinical and laboratory features of both diseases.


METHODS:Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect miR-210 expression levels in PBMCs from 35 patients with SLE, 38 patients with RA, and 35 healthy controls.


RESULTS:Compared with the healthy controls, the miR-210 expression levels were significantly increased in patients with SLE(P = 0.001) and there was increased significantly expression of miR-210 in SLE with pleuritis (Z = -2.345, P = 0.019) and anti-SSB/La-positive group (Z = -2.076, P = 0.038). However, we have not found the significant correlation between the miR-210 levels and the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score (r s = 0.091, P = 0.602). Although, no significant difference between miR-210 levels in RA patients and those in healthy controls was found (Z = -1.226, P = 0. 220). There was a significant decreased expression of miR-210 in active RA patients than inactive RA patients (Z = -4.011, P < 0.001).


CONCLUSIONS:The dysregulation of miR-210 levels in SLE and RA patients suggests that miR-210 might play an important role in the pathogenesis of these diseases.


引自: Ir J Med Sci. 2017 May 30.

 

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